Wickerhamomyces anomalus 95 shtammining fitopatogen zamburug‘larga qarshi antagonist potensiali va uchuvchan metabolitlar profili

Antagonistic potential of Wickerhamomyces anomalus strain 95 against phytopathogenic fungi and its volatile metabolite profile

Main Article Content

Nizomova Dildora Komil kizi
Juraeva Rohila Nazarovna

Abstract

This study evaluated the antagonistic activity of native Wickerhamomyces anomalus yeast strains against phytopathogenic fungi (Penicillium expansum, Aspergillus flavus, Fusarium oxysporum). Screening results showed that W. anomalus strains 120 and 95 exhibited the highest antagonistic efficacy (87,2–88,3%). Gas chromatography–mass spectrometry (GC-MS) analysis of the W. anomalus 95 extract revealed 38 metabolites, including 2-phenylethanol and phenolic compounds with pronounced antifungal properties. These findings confirm the potential of developing effective biofungicides based on these strains for postharvest protection of agricultural products.

Article Details

Section

Fundamental and Applied Microbiology

How to Cite

Nizomova Dildora Komil kizi, & Juraeva Rohila Nazarovna. (2026). Antagonistic potential of Wickerhamomyces anomalus strain 95 against phytopathogenic fungi and its volatile metabolite profile. Partner Conferences of the International Scientific Journal Research Focus, 1(8), 27-32. https://doi.org/10.66073/ReFocus-Conf/3010003

References

Olana RC, Harishchandra DL, Haituk S, et al. (2026) Mycofumigation with Beneficial Yeasts: An Eco-Friendly Approach Against Postharvest Pathogens. Agronomy 16: 392. DOI: https://doi.org/10.3390/agronomy16030392

Yang Q, Lanhuang B, Zhu M, et al. (2023) Biocontrol efficacy of Wickerhamomyces anomalus on tomato field diseases and study of the relevant mechanisms via microbiome analysis. Biological Control 184: 105285. DOI: https://doi.org/10.1016/j.biocontrol.2023.105285

Lanhuang B, Yang Q, Godana EA, et al. (2022) Efficacy of the Yeast Wickerhamomyces anomalus in Biocontrol of Gray Mold Decay of Tomatoes and Study of the Mechanisms Involved. Foods 11: 720. DOI: https://doi.org/10.3390/foods11050720

Wang K, Ngea GLN, Godana EA, et al. (2023) Recent advances in Penicillium expansum infection mechanisms and current methods in controlling P. expansum in postharvest apples. Critical Reviews in Food Science and Nutrition 63: 2598–2611. DOI: https://doi.org/10.1080/10408398.2021.1978384

Raynaldo FA, Dhanasekaran S, Ngea GLN, et al. (2021) Investigating the biocontrol potentiality of Wickerhamomyces anomalus against postharvest gray mold decay in cherry tomatoes. Scientia Horticulturae 285: 110137. DOI: https://doi.org/10.1016/j.scienta.2021.110137

Freimoser FM, Rueda-Mejia MP, Tilocca B, et al. (2019) Biocontrol yeasts: mechanisms and applications. World J Microbiol Biotechnol 35: 154. DOI: https://doi.org/10.1007/s11274-019-2728-4

Zhang H, Godana EA, Sui Y, et al. (2020) Biological control as an alternative to synthetic fungicides for the management of grey and blue mould diseases of table grapes: a review. Critical Reviews in Microbiology 46: 450–462. DOI: https://doi.org/10.1080/1040841X.2020.1794793

Godana EA, Edo GS, Yang Q, et al. (2024) Wickerhamomyces anomalus: A promising yeast for controlling mold growth and diverse biotechnological applications. Trends in Food Science & Technology 151: 104649. DOI: https://doi.org/10.1016/j.tifs.2024.104649

Cappelli A, Ulissi U, Valzano M, et al. (2014) A Wickerhamomyces anomalus Killer Strain in the Malaria Vector Anopheles stephensi. PLoS ONE 9: e95988. DOI: https://doi.org/10.1371/journal.pone.0095988

Zhang Q, Zhao L, Li Z, et al. (2019) Screening and identification of an antagonistic yeast controlling postharvest blue mold decay of pears and the possible mechanisms involved. Biological Control 133: 26–33. DOI: https://doi.org/10.1016/j.biocontrol.2019.03.002

Parafati L, Cirvilleri G, Restuccia C, et al. (2017) Potential Role of Exoglucanase Genes (WaEXG1 and WaEXG2) in the Biocontrol Activity of Wickerhamomyces anomalus. Microb Ecol 73: 876–884. DOI: https://doi.org/10.1007/s00248-016-0887-5

Zepeda-Giraud LF, Olicón-Hernández DR, Pardo JP, et al. (2020) Biological Control of Thielaviopsis paradoxa and Colletotrichum gloeosporioides by the Extracellular Enzymes of Wickerhamomyces anomalus. Agriculture 10: 325. DOI: https://doi.org/10.3390/agriculture10080325

Hong S-H, Song Y-S, Seo D-J, et al. (2017) Antifungal activity and expression patterns of extracellular chitinase and β-1,3-glucanase in Wickerhamomyces anomalus EG2 treated with chitin and glucan. Microbial Pathogenesis 110: 159–164. DOI: https://doi.org/10.1016/j.micpath.2017.06.038

Zhang X, Yang Q, Solairaj D, et al. (2024) Volatile Organic Compounds of Wickerhamomyces anomalus Prevent Postharvest Black Spot Disease in Tomato. Foods 13: 1949. DOI: https://doi.org/10.3390/foods13121949

Amorim-Rodrigues M, Brandão RL, Cássio F, et al. (2025) Wickerhamomyces anomalus is a predator of the Castanea spp. ink disease-causing oomycetes Phytophthora cinnamomi and P. xcambivora. Based on morphological evidence, a model illustrating a specific mode of action is provided. Fungal Biology 129: 101663. DOI: https://doi.org/10.1016/j.funbio.2025.101663

Wu F, Lin Y, Zheng B, et al. (2024) 2-Phenylethanol biocontrol postharvest tomato gray mold and its effect on tomato quality. Scientia Horticulturae 337: 113550. DOI: https://doi.org/10.1016/j.scienta.2024.113550

Contarino R, Brighina S, Fallico B, et al. (2019) Volatile organic compounds (VOCs) produced by biocontrol yeasts. Food Microbiology 82: 70–74. DOI: https://doi.org/10.1016/j.fm.2019.01.008

Zhao Q, Zhang Y, Solairaj D, et al. (2025) A novel volatile organic compound composite from Wickerhamomyces anomalus controls blue and gray mold of kiwifruit through direct inhibition and host resistance activation. Food Control 175: 111309. DOI: https://doi.org/10.1016/j.foodcont.2025.111309