Paenibacillus sp. bakteriyasida ekzopolisaxarid hosil bo‘lishi va antifungal faollikning EPS ajratilgandan keyingi fraksiyada aniqlanishi

Exopolysaccharide production by Paenibacillus sp. and detection of antifungal activity in the fraction remaining after EPS separation

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Published: 2026-10-30
DOI: https://doi.org/10.66073/ReFocus-Conf/3010011
Keywords:
Paenibacillus sp., Fusarium oxysporum f. sp. vasinfectum, antifungal activity, exopolysaccharide, post-EPS fraction, biological control

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Begmatov Jorabek
Institute of Genetics and Plant Experimental Biology of the Academy of Sciences of the Republic of Uzbekistan
Aytenov Ilham Sarsenbay uli
Institute of Genetics and Plant Experimental Biology of the Academy of Sciences of the Republic of Uzbekistan
Bazarbaeva Sapargul
Institute of Genetics and Plant Experimental Biology of the Academy of Sciences of the Republic of Uzbekistan
Bozorov Tohir
Institute of Genetics and Plant Experimental Biology of the Academy of Sciences of the Republic of Uzbekistan

Abstract

This study investigated the growth of Paenibacillus sp. in various liquid nutrient media and the preliminary detection of fractions exhibiting antifungal activity against Fusarium oxysporum f. sp. vasinfectum. The bacterium was cultivated in Nutrient Broth (NB), Luria–Bertani (LB), Potato Dextrose (PD), Tryptone Soy Broth (TSB), ISP2 and Corn Steep Liquor (CSL) media at 28 °C and 150 rpm for 5 days. Preliminary testing of the resulting extracts revealed no significant antifungal effect. Exopolysaccharide (EPS) production was observed in ISP2 medium, and the EPS was separated using ethanol. When tested separately, the EPS fraction did not inhibit the growth of F. oxysporum f. sp. vasinfectum, whereas the fraction remaining after EPS separation suppressed the growth of the fungal mycelium. The results indicate that the active compounds need to be isolated by chromatographic methods and their chemical nature determined in subsequent stages of the work.

Article Details

Issue

Vol. 1 No. 8 (2026): Status and Development Prospects of Fundamental and Applied Microbiology: The Viewpoint of Young Scientists

Section

Fundamental and Applied Microbiology
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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Begmatov Jorabek, Aytenov Ilham Sarsenbay uli, Bazarbaeva Sapargul, & Bozorov Tohir. (2026). Exopolysaccharide production by Paenibacillus sp. and detection of antifungal activity in the fraction remaining after EPS separation. Partner Conferences of the International Scientific Journal Research Focus, 1(8), 60-61. https://doi.org/10.66073/ReFocus-Conf/3010011

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