Bacillus thuringiensis Bt1Fo shtammining tanlangan cry1Ia geni uchun mutagen praymerlarni loyihalash va PCR tahlili

Design of mutagenic primers for the selected cry1Ia gene of Bacillus thuringiensis strain Bt1Fo and PCR analysis

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Published: 2026-10-30
DOI: https://doi.org/10.66073/ReFocus-Conf/3010039
Keywords:
Bacillus thuringiensis, Cry1Ia, Bt1Fo, primer design, PCR, mutagenesis

Main Article Content

Reyimberdiyeva Marjon Zafar kizi
Tashkent Institute of Chemical Technology
Karimov Nurbek Rayimovich
National University of Uzbekistan named after Mirzo Ulugbek
Rahimova Sh.Sh.
Tashkent Institute of Chemical Technology
Kobilov Fazliddin Bozorovich
Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan

Abstract

Insecticidal proteins of the Cry family, produced by Bacillus thuringiensis, play an important role in the biological control of agricultural pests. Previous studies have demonstrated the activity of certain Cry1Ia protein variants against the sugarcane giant borer, Telchin licus. This study aimed to design primers targeting amino acid substitutions previously described in the literature, based on the cry1Ia gene from B. thuringiensis strain Bt1Fo (GenBank accession number: PP897829), and to perform a preliminary assessment of PCR amplification results. Primers corresponding to the D233N, I116T, L266F, and E639G substitutions were selected, and PCR amplification was performed. Gel electrophoresis revealed prominent DNA bands in all four samples. These findings provide preliminary evidence of PCR product generation.

Article Details

Issue

Vol. 1 No. 8 (2026): Status and Development Prospects of Fundamental and Applied Microbiology: The Viewpoint of Young Scientists

Section

Biotechnology, Molecular Biology and Bioinformatics
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Reyimberdiyeva Marjon Zafar kizi, Karimov Nurbek Rayimovich, Rahimova Sh.Sh., & Kobilov Fazliddin Bozorovich. (2026). Design of mutagenic primers for the selected cry1Ia gene of Bacillus thuringiensis strain Bt1Fo and PCR analysis. Partner Conferences of the International Scientific Journal Research Focus, 1(8), 155-159. https://doi.org/10.66073/ReFocus-Conf/3010039

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