Bug‘doyning qurg‘oqchilikka chidamliligini in vitro sharoitida baholash

Assessment of drought tolerance in wheat under in vitro conditions

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Published: 2026-10-30
DOI: https://doi.org/10.66073/ReFocus-Conf/3010042
Keywords:
Wheat, in vitro, explant, immature embryo, callusogenesis, drought stress

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Umarova Gulnoza Abdurashidovna
Andijan State University named after Zahiriddin Muhammad Babur
Babayeva S.S.
Institute of Genetics and Plant Experimental Biology of the Academy of Sciences of the Republic of Uzbekistan
Kimsanova G.A.
Andijan State University named after Zahiriddin Muhammad Babur

Abstract

This article highlights the effectiveness of using in vitro laboratory methods and tissue cultures for selecting wheat varieties resistant to drought stress. In this study, immature wheat embryos were used as explants, and the processes of embryogenic callus formation under the influence of 2,4-D on MS nutrient medium and its subsequent regeneration were evaluated. To simulate drought stress in the selective medium, PEG compounds (10%, 20%) and mannitol (10%) were used as osmotic agents. The results demonstrate the high efficiency of the cell and tissue culture method for rapidly assessing the resistance of genotypes to osmotic stress.

Article Details

Issue

Vol. 1 No. 8 (2026): Status and Development Prospects of Fundamental and Applied Microbiology: The Viewpoint of Young Scientists

Section

Biotechnology, Molecular Biology and Bioinformatics
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Umarova Gulnoza Abdurashidovna, Babayeva S.S., & Kimsanova G.A. (2026). Assessment of drought tolerance in wheat under in vitro conditions. Partner Conferences of the International Scientific Journal Research Focus, 1(8), 167-170. https://doi.org/10.66073/ReFocus-Conf/3010042

References

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Бычкова, О. В., Ерещенко, Д. В., & Розова, М. А. (2016). Сравнительная оценка использования зрелых и незрелых зародышей яровой твердой пшеницы в культуре in vitro. Acta Biologica Sibirica, 2(2), 76-80.

Ismayilova, G., Firdovsi, K. S., & Heybat, M. M. Responses of wheat genotypes to different concentrations of 2, 4-d during in vitro cultivation of immature embryos. In The XIV International Scientific and Practical Conference «Current problems of mankind and ways to solve them», December 02-04, 2024, Munich, Germany. 439 p. Text Copyright© 2024 by the European Conference (https://eu-conf. com/). Illustrations© 2024 by the European Conference. Cover design: European Conference (https://eu-conf. com/). (p. 14).

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