Bacillus subtilisda rekombinant oqsil sekretsiyasini optimallashtirish
Optimization of recombinant protein secretion in Bacillus subtilis
Article Sidebar
Main Article Content
Abstract
Approaches to improving recombinant protein secretion in Bacillus subtilis are reviewed. Interactions between signal peptides, promoters, ribosome binding sites and host strain properties are examined. The literature highlights the need to coordinate protein synthesis, folding and membrane translocation to improve productivity.
Article Details
Section
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
References
Vološen, T. et al. A novel signal peptide toolbox for optimized heterologous expression of yeast pheromones in Bacillus subtilis. Journal of Genetic Engineering and Biotechnology 2025, 23, 100551, DOI:10.1016/j.jgeb.2025.100551. DOI: https://doi.org/10.1016/j.jgeb.2025.100551
Freudl R. Signal peptides for recombinant protein secretion in bacterial expression systems. Microb Cell Fact. 2018;17:52. DOI: 10.1186/s12934-018-0901-3. DOI: https://doi.org/10.1186/s12934-018-0901-3
Brockmeier U. et al. Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria. J Mol Biol. 2006;362:393–402. DOI: 10.1016/j.jmb.2006.07.034. DOI: https://doi.org/10.1016/j.jmb.2006.07.034
Volkenborn K. et al. The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis. Microb Cell Fact. 2020;19:154. DOI: 10.1186/s12934-020-01404-2. DOI: https://doi.org/10.1186/s12934-020-01404-2
Li D. et al. High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components. Microb Cell Fact. 2019;18:17. DOI: 10.1186/s12934-019-1066-4. DOI: https://doi.org/10.1186/s12934-019-1066-4
Li Y. et al. A genetic toolkit for efficient production of secretory protein in Bacillus subtilis. Bioresour Technol. 2022;363:127885. DOI: 10.1016/j.biortech.2022.127885. DOI: https://doi.org/10.1016/j.biortech.2022.127885
Westers H. et al. The CssRS two-component regulatory system controls a general secretion stress response in Bacillus subtilis. FEBS J. 2006;273:3816–3827. DOI: 10.1111/j.1742-4658.2006.05389.x. DOI: https://doi.org/10.1111/j.1742-4658.2006.05389.x
Teufel F. et al. SignalP 6.0 predicts all five types of signal peptides using protein language models. Nat Biotechnol. 2022;40:1023–1025. DOI: 10.1038/s41587-021-01156-3. DOI: https://doi.org/10.1038/s41587-021-01156-3
Grasso S. et al. Signal peptide efficiency: from high-throughput data to prediction and explanation. ACS Synth Biol. 2023;12:390–404. DOI: 10.1021/acssynbio.2c00328. DOI: https://doi.org/10.1021/acssynbio.2c00328