ITS-based identification and phylogenetic characterization of endophytic Alternaria sp. H8 isolated from Helichrysum mussae

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Published: 2026-10-30
DOI: https://doi.org/10.66073/ReFocus-Conf/3010053
Keywords:
endophytic fungi, Helichrysum mussae, Alternaria, ITS sequencing, fungal identification, phylogenetic analysis

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Ubaydullaev Ziyokhoja
Chirchik State Pedagogical University
Khujanov Alisher
Samarkand State University named after Sharof Rashidov
Fayziev Vokhid Bakhramovich
Chirchik State Pedagogical University
Madaminov Sulaymon
Institute for Advanced Studies at New Uzbekistan University
Eshboev Farhod Bakir ugli
Institute for Advanced Studies at New Uzbekistan University

Abstract

An endophytic fungal isolate, H8, recovered from Helichrysum mussae, was investigated using internal transcribed spacer (ITS) sequence analysis to clarify its taxonomic placement. A quality-trimmed, 544-bp reverse-strand ITS read was compared with reference sequences of five Alternaria type strains. Phylogenetic analysis placed H8 within the genus Alternaria. The isolate matched the A. destruens type strain across 521 aligned positions and shared 99.4–99.8% sequence identity with four additional species. Because the ITS region showed limited variation among the reference taxa, these findings support identification at the genus level but do not establish a definitive species assignment. Additional multilocus sequencing is required for species-level resolution.

Article Details

Issue

Vol. 1 No. 8 (2026): Status and Development Prospects of Fundamental and Applied Microbiology: The Viewpoint of Young Scientists

Section

Biotechnology, Molecular Biology and Bioinformatics
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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Ubaydullaev Ziyokhoja, Khujanov Alisher, Fayziev Vokhid Bakhramovich, Madaminov Sulaymon, & Eshboev Farhod Bakir ugli. (2026). ITS-based identification and phylogenetic characterization of endophytic Alternaria sp. H8 isolated from Helichrysum mussae. Partner Conferences of the International Scientific Journal Research Focus, 1(8), 198-199. https://doi.org/10.66073/ReFocus-Conf/3010053

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