Identification and phylogenetic placement of an endophytic Alternaria isolate D15 from Ferula khustanica

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Published: 2026-10-30
DOI: https://doi.org/10.66073/ReFocus-Conf/3010055
Keywords:
Alternaria, Ferula khustanica, endophytic fungi, ITS sequencing, molecular identification, phylogenetic analysis

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Khamdamov Doniyor Shukhratovich
Institute for Advanced Studies at New Uzbekistan University
Eshboev Farhod Bakir ugli
Institute for Advanced Studies at New Uzbekistan University

Abstract

An endophytic fungal isolate, D15, recovered from Ferula khustanica (Apiaceae), was investigated to establish its taxonomic placement using internal transcribed spacer (ITS) sequencing. A 582-base reverse-strand Sanger read was compared with reference sequences from five Alternaria type strains, and a UPGMA phylogenetic tree was constructed. The isolate clustered within Alternaria and showed 99.2–99.8% sequence identity to the examined type strains. However, the low ITS divergence among these reference species prevents a reliable species-level assignment. The isolate is therefore designated Alternaria sp. D15 pending multilocus characterization.

Article Details

Issue

Vol. 1 No. 8 (2026): Status and Development Prospects of Fundamental and Applied Microbiology: The Viewpoint of Young Scientists

Section

Biotechnology, Molecular Biology and Bioinformatics
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Khamdamov Doniyor Shukhratovich, & Eshboev Farhod Bakir ugli. (2026). Identification and phylogenetic placement of an endophytic Alternaria isolate D15 from Ferula khustanica. Partner Conferences of the International Scientific Journal Research Focus, 1(8), 203-204. https://doi.org/10.66073/ReFocus-Conf/3010055

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