Identification and phylogenetic placement of the fungal isolate S14 from Silybum marianum within Aspergillus section Nigri

Main Article Content

Khayrullaeva Lobar Mansur kizi
Umarova Gulbakhor
Eshboev Farhod Bakir ugli

Abstract

Endophytic fungal isolate S14 from Silybum marianum was examined using a 340-bp ITS sequence and compared with reference sequences of Aspergillus section Nigri type strains. Phylogenetic analysis placed S14 within section Nigri, with 98.5% sequence identity to five closely related type strains. Because the analyzed ITS region did not discriminate among these species, the isolate was designated Aspergillus sp. (section Nigri) S14 pending confirmatory sequencing and analysis of additional loci.

Article Details

Section

Biotechnology, Molecular Biology and Bioinformatics

How to Cite

Khayrullaeva Lobar Mansur kizi, Umarova Gulbakhor, & Eshboev Farhod Bakir ugli. (2026). Identification and phylogenetic placement of the fungal isolate S14 from Silybum marianum within Aspergillus section Nigri. Partner Conferences of the International Scientific Journal Research Focus, 1(8), 205-206. https://doi.org/10.66073/ReFocus-Conf/3010056

References

Bian C., Kusuya Y., Sklenář F. et al. Reducing the number of accepted species in Aspergillus series Nigri // Studies in Mycology. – 2022. – Vol. 102. – P. 95–132. – DOI: 10.3114/sim.2022.102.03. DOI: https://doi.org/10.3114/sim.2022.102.03

El-Elimat T., Raja H.A., Graf T.N. et al. Flavonolignans from Aspergillus iizukae, a fungal endophyte of milk thistle (Silybum marianum) // Journal of Natural Products. – 2014. – Vol. 77, No. 2. – P. 193–199. – DOI: 10.1021/np400955q. DOI: https://doi.org/10.1021/np400955q

Samson R.A., Visagie C.M., Houbraken J. et al. Phylogeny, identification and nomenclature of the genus Aspergillus // Studies in Mycology. – 2014. – Vol. 78. – P. 141–173. – DOI: 10.1016/j.simyco.2014.07.004. DOI: https://doi.org/10.1016/j.simyco.2014.07.004

Shah W.H., Khan W., Nisa S. et al. HPLC, NMR Based Characterization, Antioxidant and Anticancer Activities of Chemical Constituents from Therapeutically Active Fungal Endophytes // Journal of Microbiology and Biotechnology. – 2024. – Vol. 34, No. 7. – P. 1452–1463. – DOI: 10.4014/jmb.2403.03036. DOI: https://doi.org/10.4014/jmb.2403.03036