Cassia acutifolia Delile uruğlarini aseptik şaroitda in vitro kulturaga introduksiya qiliş texnologiyasi

Abstract

The article presents the results of research on developing a technology for the aseptic introduction of Cassia acutifolia Delile seeds into in vitro culture. The aim of the study is to determine effective conditions for seed sterilization and their subsequent cultivation under aseptic conditions in order to obtain viable primary plant material. The work examined variants of pre-sterilization treatment and sterilization of seeds, as well as the conditions for placing them on a nutrient medium and growing them in vitro. The level of contamination, the seed germination percentage, the viability of the obtained seedlings and the timing of their development were evaluated. As a result, the most effective conditions ensuring the production of aseptic and viable plant material of Cassia acutifolia Delile were identified. The results obtained can be used for further propagation of the plant in in vitro culture, for research on morphogenesis and for developing methods of obtaining planting material.